Leishmania Donovani Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0632-ZJF)

The kit uses SYBR Green real-time PCR to detect Leishmania Donovani by amplifying its conserved gene segments. The specific primers and optimized reaction mix enable efficient amplification and signal generation. Fluorescence is captured in real time by the PCR instrument, facilitating sensitive and accurate analysis of the target nucleic acid.

Kit Components:
Leishmania Donovani Amplification Mix: 50 µL × 1 tube
Leishmania Donovani Reaction Mix: 950 µL × 1 tube
Leishmania Donovani Positive Control: 50 µL × 1 tube
Leishmania Donovani Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Plug-and-play PCR kit—no extra preparation needed.
High target fidelity thanks to conserved sequence alignment.
Maximum sensitivity with minimized background noise.
Comes with benchmark positive control for result assurance.
One-step fluorescence PCR avoids contamination.
For non-clinical research use only.

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