JAK/STAT Pathway Assay (CAT#: STEM-MB-0338-WXH)

The mechanism of JAK-STAT signaling pathway is that cytokines bind to receptors and cause receptor dimerization, so that JAK kinases coupled with receptors approach each other and are activated by tyrosine phosphorylation. The activated JAK kinase then catalyzes the phosphorylation of the tyrosine residues of the cytokine receptor itself and forms the site where STAT binds to the receptor complex. STAT binds to the activated receptor through its SH2 domain and is Phosphorylation is achieved under the catalysis of activated JAK kinase. Phosphorylated STAT forms homo/heterodimers and enters the cell nucleus, combines with the corresponding target gene promoter region to activate the transcription and expression of the corresponding gene.

To study the regulation mechanism of JAK-STAT cell signal pathway in disease
To study the effect of each virus on JAK-STAT cell signaling pathway
To study the effects of drugs or therapies on JAK-STAT cell signaling pathways

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: JAK, SHP-1, SHP-2, SOCS3, SOCS, Src, E-Ras, Raf, Stat3, Stat, MEK, Erk, Akt, PIAS, PTP, p120, PI3K, mTOR, TF, Shc, GRB2