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Investigation of chromosome inner structure by electron tomography and electron diffraction in a transmission electron microscope (CAT#: STEM-MIT-0011-LJX)

Introduction

Chromosomes are the specific forms of DNA present in cells during mitosis or meiosis. They are formed by spiral coils of chromatin filaments that gradually shorten and thicken.<br />Chromosomes are species-specific, and their number, size and morphology vary with species, cell type and developmental stage.




Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Chromosome

Notes

Pay attention to air humidity
Voltage needs to be stabilized