Immobilized Biotin, Spacer Arm 9Å, 5ml Resin, 786-598, G-BIOSCIENCES (CAT#: STEM-C-3120-LGZ)

Immobilized biotin resin for high affinity chromatography purification of avidin, streptavidin and neutravidin proteins. The resin consists of biotin conjugated to 6% cross-linked agarose.
Biotin is a 244 Dalton vitamin (vitamin H) molecule that exhibits extraordinary binding affinity for avidin (Ka=1015M-1) and streptavidin. The biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-HCl and extreme pH. The biotin-avidin bond can only be reversed by denaturing the avidin protein molecule with 8M guanidine-HCl at pH 1.5 or by boiling in SDS PAGE sample loading buffer.

Size: 5ml resin

Strong affinity for avidin, streptavidin and neutravidin
Reusable resin, at least 10 times
Covalently coupled to limit leaching
Spacer arm: 9Å

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