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Imaging of adenine and thymine at atomic resolution by the scanning tunneling microscope (CAT#: STEM-MIT-0321-LJX)

Introduction

Adenine and thymine are the two bases. The bases are nitrogenous compounds that form nucleosides. The bases are found in an organism's genetic material, DNA or RNA.<br />The scanning tunneling microscope has been used to obtain images of DNA that reveal its major and minor grooves and the direction of helical coiling, and sufficient resolution can be achieved to identify its bases.




Principle

The basic principle of scanning tunneling microscope (STM) is to use the tunneling effect in quantum theory. An extremely fine probe of atomic linearity and the surface of the substance under study are used as two electrodes. When the sample is very close to the tip of the needle (usually less than 1nm), electrons flow through the barrier between the two electrodes to the other electrode under the action of an applied electric field. This phenomenon is known as tunneling effect.

Applications

Studing the structure of the sample surface by high resolution imaging
Manipulating individual atoms to form nanostructures

Procedure

1. Sampling
2. Preparation of slices (Select according to the specific experimental situation)
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Adenine and thymine attached to the basal plane of highly oriented pyrolytic graphite

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures