IL-9 Detection (CAT#: STEM-MB-0278-WXH)

Introduction

Interleukin-9 is released by helper T cells and can also be produced by T cells transformed by T cell leukemia virus 1 (HTLV-1), which is a type of T cell growth factor. It is expressed in human eosinophils, natural killer cells, Th9 cells, Th17 cells and Treg cells. In addition to stimulating the growth of T cells, it can also support the growth of hematopoietic progenitor cells, expand the response of bone marrow mast cells to IL-3, and promote various functions such as the growth of certain leukemia cell lines, thymoma cell lines, and T cells. IL-9 can stimulate airway epithelial cells (AEC) to secrete certain proteins and activate the expression of AEC mucin gene subsets, causing AEC to secrete excessive mucusIL-9 can also directly act on AEC to produce chemokines and promote the accumulation of inflammatory cells in the lungs, which is related to human asthma.

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Principle Applications Procedure Materials Notes Related Products

Principle

IL-9 acts through a receptor on the surface of the target cell membrane. The receptor for IL-9 (IL-9R) exists on the surface of a variety of cell membranes. It is a heterodimer structure composed of a specific α-subunit ligand and A common gamma chain consists of two strands. IL-9 can promote tumor cell proliferation by activating the STAT3 phosphorylation level and then activating the STAT3 signaling pathway. The activation of IL-9 promotes the phosphorylation of protein tyrosine kinase 3 (JAK3), thereby causing signal transduction in downstream regions and activation of transcription activation factor (STAT), especially STAT1 homodimer, STAT5 homodimer and STAT1-STAT3 heterodimer. STAT activation is necessary in IL-9-induced T cell proliferation.

Applications

IL-9 plays a role in peribronchial fibrosis, and overexpression of IL-9 can aggravate fibrosis around the bronchi.
IL-9 can directly act on red blood cell-oriented liver cells, and has the effect of selectively stimulating red blood cell production.
IL-9 is related to allergic asthma.
IL-9 can stimulate the growth of T cells.
IL-9 can also support the growth of hematopoietic progenitor cells.
IL-9 expands the response of bone marrow mast cells to IL-3
IL-9 can promote various functions such as the growth of certain leukemia cell lines, thymoma cell lines, and T cells.

Procedure

1. Process samples.
2. IL-9 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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