IGF detection service (CAT#: STEM-MB-0257-WXH)

Introduction

Insulin-like growth factor (IGF) is a group of growth-promoting peptides. Its secreting cells are widely distributed in human liver, kidney, lung, heart, brain, and intestine. The production of IGF-I is more dependent on GH, which has a strong growth-promoting effect and is an important growth factor in childhood. IGF-I synthesized in various tissues mostly exerts its growth-promoting effects in an autocrine or paracrine manner, while IGF-I synthesized by the liver enters the blood circulation and acts on target cells in an endocrine manner. The level of IGF-I in the body is regulated by GH. IGF- I also has a negative feedback regulation effect on GH secretion. IGF-II is more insulin-like and plays an important role in fetal growth. IGF receptors are divided into two types, namely, IGF-I receptor (IGF-I R) and IGF-II receptor (IGF-II R). The former is a tyrosine kinase-type receptor and is composed of two α subunits. The latter has no amino acid kinase activity.

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Principle Applications Procedure Materials Notes Related Products

Principle

At present, there are mainly two cellular signal transduction pathways, namely the RAS / RAF / MEK / ERK signal transduction pathway and the PI3K / AKT signal transduction pathway. Studies have shown that IGF-mediated mitosis and cell proliferation and differentiation are mainly accomplished through the RAS / RAF / MEK / ERK signal transduction pathway. When IGFR binds to IGF, its tyrosine and serine residues are phosphorylated, which in turn activates IGFR. The activated IGFR binds to the SH2 domain of growth factor receptor binding protein-2 (Grb2), while the SH3 domain of Grb2 can bind to the son of sevenless (SOS), which results RAS dissociates GDP and binds GTP, thereby activating RAS. Activated RAS further binds to the amino terminus of the serine / threonine protein kinase RAF-1, thereby activating RAF-1. RAF-1 activates MEK by phosphorylating MEK1 / MEK2. MEK is a bispecific kinase that can phosphorylate serine / threonine and tyrosine, and ultimately selectively activate ERK1 and ERK2. ERK is a proline-directed serine / threonine kinase that can phosphorylate serine / threonine adjacent to proline. After mitogen stimulation, ERK receives upstream cascade response signals, which can be converted into the nucleus and regulate cell proliferation and differentiation.

Applications

IGF mediates growth hormone stimulation, regulates tissue growth and development, and plays an important role in muscle volume and strength, maintenance of body composition, and regulation of nutrient metabolism.

Procedure

1. Process samples.
2. IGF detection (Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Serum, cell supernatant, urine, body fluids, lavage fluid, cerebral spinal cord, atrial fluid, pleural fluid, tissue, etc.

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