Identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles by UV-Vis Spectroscopy (CAT#: STEM-MB-0919-WXH)

Name: Identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles by UV-Vis Spectroscopy
SKU: STEM-MB-0919-WXH
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Introduction

Superparamagnetic iron oxide nanoparticles (SPIONs) are a type of MNP that show magnetic properties in the presence of an external magnetic field. Superparamagnetic iron oxide nanoparticles (SPIONs) are increasingly used in medical applications, such as targeting delivery and imaging. In the future, patients are more likely to be exposed to pharmaceutical products containing such particles.

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Principle Applications Procedure Materials

Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.