Human Leukocyte Antigen A (HLA-A) Gene Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0063-ZJF)

Utilizing SYBR Green real-time PCR technology, this detection kit enables the amplification and quantification of Human Leukocyte Antigen A (HLA-A) Gene with high efficiency. Specific primers are designed to bind to conserved gene regions of the target. As PCR progresses, fluorescence is generated and captured by the system in real time, providing clear and reliable interpretation of the presence and quantity of Human Leukocyte Antigen A (HLA-A) Gene in test samples.

Kit Components:
Human Leukocyte Antigen A (HLA-A) Gene Amplification Mix: 50 µL × 1 tube
Human Leukocyte Antigen A (HLA-A) Gene Reaction Mix: 950 µL × 1 tube
Human Leukocyte Antigen A (HLA-A) Gene Positive Control: 50 µL × 1 tube
Human Leukocyte Antigen A (HLA-A) Gene Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use kit requiring only user-supplied DNA template.
High specificity: Primers are designed based on highly conserved regions of the HLA-A gene, ensuring no amplification of unrelated genes.
Optimized primers and amplification system provide 100-fold greater sensitivity than conventional PCR.
Includes non-infectious PCR positive control to help distinguish false-negative results.
Closed one-tube fluorescence PCR workflow reduces the risk of environmental contamination.
For research use only; kit provides sufficient reagents for 50 PCR reactions using a 2 μL system.

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