HOOK™ 6X His Protein Spin Purification Kit (Bacteria), Nickel Resin, Size 25, 786-628, G-BIOSCIENCES (CAT#: STEM-GT-3017-LGZ)

HOOK™ 6X His Protein Spin Purification Kit provides rapid purification of soluble 6X His-tagged bacterial culture proteins.

Bacteria are first lysed with bacterial PE LB™ and PE LB™-lysozyme, releasing total soluble protein while maintaining protein structure and activity. 0.5 ml of immobilized metal affinity resin was added to the clarified lysate, and the 6X His-tagged protein was purified by immobilized metal affinity chromatography (IMAC). The resin is transferred to a convenient spin column where it is washed quickly and the 6X His protein is eluted with imidazole buffer.

Bacteria - PELB™ Kit has been developed for the extraction of soluble proteins from bacterial cells. It is based on a patented improvement of lysozyme lysis, allowing extraction of soluble proteins while removing nucleic acids (DNA and RNA) released during cell lysis. Bacteria-pe LB™ Lysis eliminates viscosity build-up, allowing efficient clarification with lower centrifugal forces. Ultrasound examination is not required.

HOOK™ 6X His Protein Spin Purification Kit can be used for immobilized nickel chelate resin or cobalt chelate resin for metal affinity chromatography. Cobalt chelate resins have a lower binding affinity for 6X His tags compared to nickel chelate resins, which results in less non-specific binding and may result in slightly lower yields.

The HOOK™ 6X His Protein Spin Purification Kit is optimized to yield ~1mg/50ml of soluble His-tagged protein with a purity of 80-90%, depending on the protein's expression level, resin type, conformation, and solubility characteristics.

For larger scale bacterial protein isolations, we recommend our gravity flow kit to extract 10mg of protein from ~250ml of bacteria.

Size: 25

Isolate >1mg His tagged proteins from ~50ml bacterial culture
No sonication required, supplied with lysis buffer and enzyme
Nickel or Cobalt resins available
Spin column format

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