HIV-1 Provirus Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0708-ZJF)

Designed for the detection of HIV-1 Provirus, this kit employs SYBR Green-based real-time PCR. It uses highly specific primers to amplify conserved gene regions of the target sequence. The real-time detection system monitors fluorescence generated during amplification, allowing for accurate qualitative and quantitative evaluation of HIV-1 Provirus in various biological or food samples.

Kit Components:
HIV-1 Provirus Amplification Mix: 50 µL × 1 tube
HIV-1 Provirus Reaction Mix: 950 µL × 1 tube
HIV-1 Provirus Positive Control: 50 µL × 1 tube
HIV-1 Provirus Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Convenient direct-use format with only template DNA input needed.
Primer sequences selected for unmatched specificity to the analyte.
Improved detection limit due to optimized assay chemistry.
Internal positive control enables result interpretation.
Enclosed qPCR protocol limits exposure and contamination.
Not intended for diagnostic or therapeutic use.

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