HiScreen Capto adhere, Cytiva (CAT#: STEM-C-1457-LC)

HiScreen Capto Adhere maufactured by Cytiva is a multimodal anion exchanger resin prepacked column which is ideal for method optimization and parameter screening. This prepacked, ready-to-use column is designed for intermediate purification and polishing of monoclonal antibodies after the protein A capture step. The 4.7 mL small column volume and 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Chromatography technique: Ion Exchange Chromatography
Bed dimensions: 7.7 × 100 mm
Bed height: 100 mm
Bed volume: 4.7 ml
Column i.d.: 7.7 mm
Flow velocity1: < 600 cm/h
Pressure max. (over the packed bed during operation): 3 bar [0.3 MPa] (44 psi)
Storage: 4 to 30°C, 20% Ethanol
Pack size: 1 × 4.7 mL

Small bed volume gives fast results and minimal sample/buffer consumption
Easily connected in series to achieve 20 cm bed height.

HiScreen SP HP, Cytiva (CAT#: STEM-C-1458-LC)