His-Tagged Protein Purification Medium (IDA), Agarose Gel Microspheres, Bogen (CAT#: STEM-C-3006-LGZ)

The His-tagged protein purification medium is suitable for the purification of His-tagged fusion proteins. This product can quickly extract the His-tagged fusion protein from cell fermentation broth.

Matrix: Agarose gel microspheres
Ligand: IDA
Shape: spherical
Average particle size of medium: 90±5 μm
Metal ion loading: ≥40 μmol Ni2+/ml
Dynamic capacity: ≥40 mg His-Pro/ml wet gel (IDA)
Maximum flow rate (25°C): ≥500 cm/h
Maximum pressure: 0.3 MPa (3 bar)
pH stability: 3~13 (long time); 2~14 (short time)
Storage: 4~8 °C (20% ethanol)

1. His-tagged protein purification medium (IDA) uses IDA as a metal chelating ligand, conventionally chelating Ni2+ (often called nickel filler), and can also chelate Cu2+, Co2+, Zn2+ plasma groups according to customer needs.
2. Please note that when choosing this product, do not add reducing agents such as mercaptoethanol or dithiothreitol to the buffer, which will cause the metal ions to fall off (if you need to add reducing agent components, it is recommended to choose NTA products from Chubo Biotechnology. ).

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