Gas-producing Clostridium Bacillus Type B Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2301-ZJF)

Designed for the detection of Gas-producing Clostridium Bacillus Type B, this kit applies probe-based real-time PCR technology. It uses primers targeting conserved gene sequences of Gas-producing Clostridium Bacillus Type B, enabling efficient amplification and fluorescent signal generation. Real-time monitoring of fluorescence ensures reliable qualitative and quantitative evaluation of the target nucleic acid.

Kit Components:
Gas-producing Clostridium Bacillus Type B Amplification Mix: 50 µL × 1 tube
Gas-producing Clostridium Bacillus Type B Reaction Mix: 950 µL × 1 tube
Gas-producing Clostridium Bacillus Type B Positive Control: 50 µL × 1 tube
Gas-producing Clostridium Bacillus Type B Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

No reagent mixing required—just input DNA to begin.
High specificity through design focused on conserved genetic loci.
Excellent signal response with optimized probes and primers.
Built-in positive control ensures dependable results.
Enclosed-tube qPCR minimizes environmental contamination.
Optimized for fast turnaround time.
Not approved for clinical use—research only.

STEM-SLSR-2299-ZJF
STEM-SLSR-2300-ZJF
STEM-SLSR-2302-ZJF
STEM-SLSR-2303-ZJF