G-Trap™ SP Agarose Fast Flow, 1 ml, 5 Columns, 786-1016, G-BIOSCIENCES (CAT#: STEM-C-3314-LGZ)

G-Trap™ SP Agarose Fast Flow is ready-to-use prepacked columns for the separation of proteins and other charge-based biomolecules. The resin in G-Trap™ SP Sepharose Fast Flow is a strong cation exchanger consisting of highly cross-linked 6% Sepharose beads plus thiopropyl (SP) strong cation exchange groups. Proteins with a net positive charge will bind to the resin, while proteins with a net negative charge will not bind and will flow out.

There are other G-Trap™ Ion Exchange Agarose Fast Flow columns available in 1 mL and 5 mL column volumes, namely G-Trap™ CM Agarose, G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast flow. G-Trap™ CM Sepharose Express contains cation exchange resin and G-Trap™ DEAE Sepharose Express and G-Trap™ Q Sepharose Express contains anion exchange resin. The G-Trap™ Ion Exchange Selection Kit can be used for initial screening of suitable ion exchangers for purification of target proteins.

G-Trap™ Ion Exchange Agarose Fast Flow Columns can separate biomolecules on the basis of charge using prepacked columns. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method detection, separation or purification of biomolecules, sample concentration and sample cleanup of charged molecules. Biomolecules carrying a net charge opposite to the ion exchanger bind to the ion exchanger and are thus separated from the bulk molecules in the sample.

Size: 5 columns

G-Trap™ SP Agarose Fast Flow resin

Ion Exchanger: Strong cation exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand: Sulphopropyl
Ionic Capacity: 0.18-0.25 mmol (Na+)/ml
Binding Capacity: 70 mg lysozyme /ml medium
pH stability Working Range: 2-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol containing 0.2M sodium acetate

G-Trap™ SP Agarose Fast Flow​​, 1 ml columns

Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

G-Trap™ SP Agarose Fast Flow​​, 5 ml columns

Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

Column, 20ml, 25 Columns, 786-197, G-BIOSCIENCES (CAT#-STEM-C-3334-LGZ)
Columns, 5ml, 25 Columns, 786-169, G-BIOSCIENCES (CAT#-STEM-C-3335-LGZ)
G-Trap™ 1ml FliQ FPLC Column, 1 Column, 786-1292, G-BIOSCIENCES (CAT#-STEM-C-3336-LGZ)
G-Trap™ 5ml FliQ FPLC Column, 1 Column, 786-1293, G-BIOSCIENCES (CAT#-STEM-C-3337-LGZ)
G-Trap™ 10ml FliQ FPLC Column, 1 Column, 786-1294, G-BIOSCIENCES (CAT#-STEM-C-3338-LGZ)
10.32 packing connector (1 pc), 1 Column, 786-1296, G-BIOSCIENCES (CAT#-STEM-C-3339-LGZ)