G-Trap™ DEAE Agarose Fast Flow, 1 ml, 5 Columns, 786-1012, G-BIOSCIENCES (CAT#: STEM-C-3309-LGZ)

G-Trap™ DEAE Agarose Fast Flow is a ready-to-use prepacked column for the separation of proteins and other charge-based biomolecules. The resin in G-Trap™ DEAE Agarose Fast Flow is a weak anion exchanger consisting of highly cross-linked 6% agarose beads plus diethylaminoethyl (DEAE) weak anion exchange groups. Proteins with a net negative charge will bind to the resin, while proteins with a net positive charge will not bind and will flow out.

There are other G-Trap™ Ion Exchange Agarose Fast Flow columns available in 1 mL and 5 mL column volumes namely G-Trap™ CM Agarose Fast Flow, G-Trap™ SP Agarose Fast Flow, G-Trap™ Q Agar Sugar rush. G-Trap™ CM Sepharose Express and G-Trap™ SP Sepharose Express contain cation exchange resin and G-Trap™ Q Sepharose Express contain anion exchange resin. G-Trap™ Ion Exchange Selection Kitis can be used for initial screening of suitable ion exchangers for purification of target proteins.

G-Trap™ Ion Exchange Agarose Fast Flow Columns can separate biomolecules on the basis of charge using prepacked columns. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method detection, separation or purification of biomolecules, sample concentration and sample cleanup of charged molecules. Biomolecules carrying a net charge opposite to the ion exchanger bind to the ion exchanger and are thus separated from the bulk molecules in the sample.

Size: 5 columns

G-Trap™ DEAE Agarose Fast Flow resin

Ion Exchanger: Weak anion exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand: Diethylaminoethyl
Ionic Capacity: 0.11-0.16mmol (Cl-)/ml
Binding Capacity: 90mg HSA/ml medium
pH stability Working Range: 2-9
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol

G-Trap™ DEAE Agarose Fast Flow ​, 1 ml columns

Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

G-Trap™ DEAE Agarose Fast Flow ​, 5 ml columns

Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

Spin Column, 10ml, 25 Columns, 786-983, G-BIOSCIENCES (CAT#-STEM-C-3329-LGZ)
Spin Column, 10ml, 50 Columns, 786-984, G-BIOSCIENCES (CAT#-STEM-C-3330-LGZ)
Spin Column, 1ml, 25 Columns, 786-810, G-BIOSCIENCES (CAT#-STEM-C-3331-LGZ)
Spin Column, 1ml, 50 Columns, 786-811, G-BIOSCIENCES (CAT#-STEM-C-3332-LGZ)
Twist Break Column, 25 Columns, 786-1810, G-BIOSCIENCES (CAT#-STEM-C-3333-LGZ)
Column, 20ml, 25 Columns, 786-197, G-BIOSCIENCES (CAT#-STEM-C-3334-LGZ)