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Fine structure of human nuclear chromatins in interphase as observed by dark field microscopy (CAT#: STEM-MIT-0336-LJX)

Introduction

The intermitotic phase is the part of the cell cycle during which a cell replicates DNA in its chromosomes and synthesizes related proteins. The chromosomes have a chromatin shape, which is long, thin filaments.




Principle

The main difference between a dark field microscope and an ordinary microscope is the way of illumination. It illuminates the specimen with a strong, narrow, slanted beam without letting the beam enter the objective lens. When no light enters the objective lens, the field of view is dark, so it is called dark field microscope. However, because the particles in the specimen can scatter light after being illuminated by light, when the scattered light enters the objective lens, the scattered light spots of the particles can be seen in the microscope, as if the particles themselves were glowing. This is just as in a dark room, through a small hole in the wall of a strong beam of sunlight, we can see the presence of dust in the light path. This phenomenon, in optics, is known as the Dundar phenomenon. The dark-field microscope is designed according to this principle.

Applications

For observing microorganisms, colloid chemistry, single-celled organisms, and objects with linear structure
Unsuitable for observing stained specimens

Procedure

1. Sample preparation
2. Assembly and adjustment of dark field microscope
3. Observation

Materials

• Sample Type:
Human nuclear chromatins

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures