Fast cycling PCR (CAT#: STEM-MB-0203-WXH)

Introduction

Fast cycling PCR is a PCR-based technology that allows amplification of specific PCR products with significantly reduced cycling time.

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Principle Applications Procedure Materials Notes Related Products

Principle

The principle in this process is the same as conventional PCR, the only difference being the time of amplification.
The buffer used in this PCR increases the affinity of Taq DNA polymerases for short single-stranded DNA fragments, reducing the time required for successful primer annealing to just 5 seconds.

Applications

Fast cycling PCR is essential for processes requiring quick cycles and also helps in the rapid diagnosis of diseases and mutations.

Procedure

General procedure:
1. Denaturation
2. Annealing
3. Extension

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