ERFG Pathway Assay (CAT#: STEM-MB-0348-WXH)

EGFR(Epidermal growth factor receptor), or ErbB1/human epidermal growth factor receptor (HER)-1, is a transmembrane protein which is the receptor of the ligands from EGF family. EGFR is a 170-kDa glycoprotein with tyrosine kinase activity. It is composed of a single polypeptide of 1186 amino acid residues that is cleaved from a 1210-residue polypeptide precursor. It has its own modular structure: it contains an extracellular ligand-binding domain, a transmembrane region and a cytoplasmic tyrosine-kinase region that is flanked by noncatalytic regulatory regions. EGFR plays a crucial role in initiating the signaling that directs the behavior of epithelial cells and tumors of epithelial origin. Human EGF is a 53-aa polypeptide, whose molecular weight is 6KDa. EGF can stimulate the cell growth and differentiation by binding to its receptor, EGFR.

To study the effect of each virus on ERFG signaling pathway
To study the regulation mechanism of ERFG signal pathway in disease
To study the effects of drugs or therapies on ERFG signaling pathways

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: PLC, PIP2, IP3, DAG, IKK, SOS, PKC, CaMK, ERK1, ERK2, MEKK1, MEKK2, Raf, Ras, GRB2, XIAP, S6K, mTOR, Akt, PDK1, PIP3, Nck, Vav, c-Cbl, Muc1, Rac1, Rho, MKK4, MKK7, p53, STAT3, JNKs, JAK1, JAK2, STAT1, STAT2, S6K, NF-kB, EIK1, c-Myc, c-Jun, c-Fos