Equine Herpesvirus Type 4 Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0833-ZJF)

This real-time PCR detection kit is based on SYBR Green fluorescence chemistry and is intended for the analysis of Equine Herpesvirus Type 4 nucleic acids. Primers targeting conserved regions enable specific amplification. During the reaction, fluorescent signals are emitted and continuously tracked by the system, providing reliable, real-time quantification and identification of the target sequence.

Kit Components:
Equine Herpesvirus Type 4 Amplification Mix: 50 µL × 1 tube
Equine Herpesvirus Type 4 Reaction Mix: 950 µL × 1 tube
Equine Herpesvirus Type 4 Positive Control: 50 µL × 1 tube
Equine Herpesvirus Type 4 Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Simplified protocol: only DNA needs to be added.
High-precision primers designed for analyte-specific detection.
Reagents optimized for robust signal sensitivity.
Positive control supports result validation and QC.
Fully enclosed PCR setup mitigates contamination risks.
Limited to academic or laboratory research.

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