Enteropathogenic Escherichia Coli (EPEC) Genus Universal Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0907-ZJF)

Optimized for high-throughput and research applications, this kit uses SYBR Green dye-based real-time PCR for the detection of Enteropathogenic Escherichia Coli (EPEC) Genus Universal. Amplification of the target gene leads to fluorescence emission, which is monitored in real time. This allows for effective and reproducible detection of target nucleic acids across a range of experimental conditions.

Kit Components:
Enteropathogenic Escherichia Coli (EPEC) Genus Universal Amplification Mix: 50 µL × 1 tube
Enteropathogenic Escherichia Coli (EPEC) Genus Universal Reaction Mix: 950 µL × 1 tube
Enteropathogenic Escherichia Coli (EPEC) Genus Universal Positive Control: 50 µL × 1 tube
Enteropathogenic Escherichia Coli (EPEC) Genus Universal Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

No setup required—add DNA and begin.
Superior specificity derived from conserved sequence primer design.
Designed for heightened assay sensitivity.
Positive control ensures detection integrity.
One-tube closed system improves contamination control.
Use limited to non-clinical research.

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