Embryo Lethal Orphan Virus Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1002-ZJF)

Highlights

Plug-and-play PCR kit—no extra preparation needed.
High target fidelity thanks to conserved sequence alignment.
Maximum sensitivity with minimized background noise.

Cat Number: STEM-SLSR-1002-ZJF

Application: For qualitative and quantitative analysis of Embryo Lethal Orphan Virus

Model: 24230




Description

This assay kit applies SYBR Green dye-based real-time PCR to amplify and detect Embryo Lethal Orphan Virus nucleic acids. Specific primers are designed to target conserved regions of the target gene. Fluorescence signals emitted during amplification are recorded in real time, supporting accurate identification and measurement of Embryo Lethal Orphan Virus in experimental and routine laboratory settings.

Specification

Kit Components:
Embryo Lethal Orphan Virus Amplification Mix: 50 µL × 1 tube
Embryo Lethal Orphan Virus Reaction Mix: 950 µL × 1 tube
Embryo Lethal Orphan Virus Positive Control: 50 µL × 1 tube
Embryo Lethal Orphan Virus Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Features

Plug-and-play PCR kit—no extra preparation needed.
High target fidelity thanks to conserved sequence alignment.
Maximum sensitivity with minimized background noise.
Comes with benchmark positive control for result assurance.
One-step fluorescence PCR avoids contamination.
For non-clinical research use only.

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