Elizabeth Bartonella Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2448-ZJF)

Highlights

Convenient kit format with no reagent preparation—add template and run.
Target-specific probes derived from conserved DNA sequences.
Optimized for detecting trace amounts with high sensitivity.

Cat Number: STEM-SLSR-2448-ZJF

Application: For qualitative and quantitative analysis of Elizabeth Bartonella

Model: 21510




Description

This real-time PCR detection kit is based on probe technology and is designed to identify Elizabeth Bartonella through specific amplification of conserved gene regions. Fluorescent signals generated during the PCR process are detected in real time, enabling precise qualitative and quantitative assessment of Elizabeth Bartonella DNA in various sample types.

Specification

Kit Components:
Elizabeth Bartonella Amplification Mix: 50 µL × 1 tube
Elizabeth Bartonella Reaction Mix: 950 µL × 1 tube
Elizabeth Bartonella Positive Control: 50 µL × 1 tube
Elizabeth Bartonella Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Features

Convenient kit format with no reagent preparation—add template and run.
Target-specific probes derived from conserved DNA sequences.
Optimized for detecting trace amounts with high sensitivity.
Positive control available for monitoring false negatives.
Closed-tube fluorescence detection improves workflow cleanliness.
Designed for speed in high-throughput labs.
Not for medical or diagnostic purposes.

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