Edwardsiella Ictaluri Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0884-ZJF)

This assay kit applies SYBR Green dye-based real-time PCR to amplify and detect Edwardsiella Ictaluri nucleic acids. Specific primers are designed to target conserved regions of the target gene. Fluorescence signals emitted during amplification are recorded in real time, supporting accurate identification and measurement of Edwardsiella Ictaluri in experimental and routine laboratory settings.

Kit Components:
Edwardsiella Ictaluri Amplification Mix: 50 µL × 1 tube
Edwardsiella Ictaluri Reaction Mix: 950 µL × 1 tube
Edwardsiella Ictaluri Positive Control: 50 µL × 1 tube
Edwardsiella Ictaluri Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Supplied in a ready-to-use format—just add DNA template.
Primers designed from conserved regions for maximum target specificity.
High sensitivity ensured through optimized primer/probe design.
Includes positive control to distinguish false negatives.
Single-tube fluorescence PCR minimizes contamination risk.
For research use only.

STEM-SLSR-0882-ZJF
STEM-SLSR-0883-ZJF
STEM-SLSR-0885-ZJF
STEM-SLSR-0886-ZJF