Eclipse™ AF4 Asymmetric-Flow Field-Flow Fractionation System, Wyatt Technology Corporation (CAT#: STEM-LE-2017-LC)

The Eclipse™ AF4 is designed for separation, fractionation and sizing of macromolecules, nanoparticles and colloids. When used with a Wyatt DAWN® or miniDAWN™ MALS detector and Optilab® T-rEX™ refractive index detector, the Eclipse AF4 provides a uniquely versatile solution for characterization of absolute molar mass and size as well as conformation and conjugation.

Type:Asymmetric-Flow Field-Flow Fractionation
Dimensions:(WxDxH) 36 x 64 x 25 cm
Max. Flow Rate:10 mL/min (crossflow plus detector flow rate)
Particle Size:1 nm to 1000 nm
Max. Pressure:30 bar

Analysis times 10 - 30 minutes
Fritless column for aggregation-prone samples
The highest quality HPLC pumps and autosamplers from Agilent, Shimadzu, Thermo, or Waters
Couple to ICP-MS for elemental analysis of nanoparticles per size bin
Powerful separation of macromolecules and nanoparticles in size of 1 nm - 1000 nm with outstanding resolution
No stationary phase
Versatile choice of separation channels, permitting sample loads from µL to mL, including semi-preparative FFF
Optimize separation methods for enhanced resolution or size range simply by adjusting flow ratios, even programmatically during a run
Collect fractions for off-line analysis (with electron microscopy, MS, ICP-MS, ELISA, etc.)

Eclipse Mobility Tunable 2D Separator, Eclipse™ DualTec Field Flow Fractionation System