DNA shuffling to generate mutant genes (CAT#: STEM-MB-0083-WXH)

This technique depends on the homologous gene fragments can be randomly recombined by DNaseI or restriction enzymes. Up to now, DNA shuffling is a powerful tool used to increase mutagenesis librari size.

Applied to protein and small molecule pharmaceuticals, bioremediation, vaccines, gene therapy, and evolved viruses.

1. Acquiring DNA fragments for shuffling
2. Reassembly of DNaseI fragments
3. Aamplificaiton of full-length sequences