Determination of Sulfhydryl Groups in Proteins and Enzymes by Indirect Amperometric Titration (CAT#: STEM-ACT-0022-CJ)

Amperometric titration refers to a class of titrations in which the equivalence point is determined through measurement of the electric current produced by the titration reaction. It is a form of quantitative analysis.

Analytical Chemistry

Amperometry is an electrochemical technique where a small electrical voltage is applied across two electrodes through a solution. The chemical reaction during the titration causes a change in current. Amperometric titration measures the current change as titrant is added.
1. Connect the titrator to the appropriate source of line voltage and turn.
2. Remove all air from the pipette and plastic tubing.
3. Sample collection.
4. Place the sample on the titrator
5. Add buffer solution to the water sample.
6. Start the agitator by turning the switch to the appropriate sensitivity position (mg/L or µg/L).
7. Add the appropriate titrant during the titration and observe the action of the meter pointer.

• Sample: Blood; Biological Fluids; Water and wastewater; Pharmaceutical sample; Drugs & More
• Equipment: Probe or cell which contains dual platinum electrodes (biamperometric) or two dissimilar electrodes.
• Microampere meter
• Titrant dispensation device.

1. This method is generally used for the determination of metal ion present in aqueous solution.
2. The titration can usually be carried out rapidly.
3. A number of amperometric titrations could be carried out at dilutions (ca 10-4 M) at that several visual or potentiometric titrations no longer yield accurate outputs.
4. The output of the titration is independent of the features of the capillary.