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Determination of methylmercury by Atomic fluorescence spectrometry (AFS) (CAT#: STEM-ST-0143-WXH)

Introduction

Methylmercury is a very poisonous form of mercury. It forms when bacteria react with mercury in water, soil, or plants. It was used to preserve grain fed to animals. Methylmercury poisoning has occurred in people who have eaten meat from animals that ate grain that was treated with this form of mercury.




Principle

Atomic fluorescence spectroscopy (AFS) is the emission of radiation energy in the UV-visible region from gas-phase atoms that have been excited to higher energy levels by absorption of radiant energy. Usually, a flame is used to obtain the atom in a gaseous state. It is a radiative emission process that proceeds from the lowest singlet (S1) to the singlet ground state(S0).

Applications

Atomic Fluorescence Spectrometry (AFS) is an analytical technique that is primarily used to detect and quantify metals. It is an exceptionally sensitive technique that relies on exploiting the distinctive fluorescent spectra of each specific metal.

Procedure

1. Sample preparetion
2. Sample ionization
3. Detection

Materials

• Line Source
• Nebulizer-burner system
• Monochromators/Filters
• Detector, Amplifier, Readout