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Determination of ethanol content in wine by methyl orange fading spectrophotometry (CAT#: STEM-PPA-0032-LJX)

Introduction

This service determines the concentration of ethanol in wine by methyl orange fading spectrophotometry. Methyl orange was discolored by quantitative ethanol in ammonium acetate buffer medium with pH 3.2. The maximum absorption wavelength λmax= 520 nm. The concentration of ethanol in the range of 0 % to 30 % obeys Beale's law.




Principle

Acid-base indicators produce different colors in aqueous solutions of their specific pH range. The indicator is added to the standard buffer solution, and the resulting color is used as the standard colorimetric tube. The pH value of the water sample can be measured by visual colorimetric comparison with the water sample colorimetric tube with the same indicator.

Applications

For determining the approximate PH value of solutions in food production, wastewater treatment, pharmaceutical and chemical industries

Procedure

1. Add ph indicator to the standard solution, and the solution with different pH will show different colors. Use these solutions as standard colorimetric tubes
2. Add the same ph indicator to the solution to be tested, and compare the color of the solution to be tested with the standard colorimetric tube to determine the approximate ph value of the solution

Materials

• Sample Type:
Wine

Notes

After dropping the indicator into the solution, it should be gently shaken to avoid inaccurate detection.