DEAE Seplife XL Weak Anion-Exchange Medium,>80 mg BSA/mL (CAT#: STEM-BC-3156-ZJF)

This weak anion-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses highly crosslinked agarose microspheres functionalized with diethylaminoethyl groups. The weak anion-exchange ligand provides selectivity different from quaternary-ammonium media. A BSA adsorption capacity above 80 mg/mL supports productive downstream processing. The medium provides high linear flow and relatively low backpressure. Low nonspecific adsorption helps maintain product recovery. Its chemical stability supports common buffers, sodium hydroxide, urea, guanidine hydrochloride, and ethanol. The matrix withstands the stated steam-treatment conditions in water. The resin can be scaled for capture, intermediate purification, and polishing applications.

Model: DEAE Seplife XL
Appearance: White spherical gel, odorless and tasteless
Type: Weak anion-exchange medium
Matrix: Seplife XL microspheres
Ligand: Diethylaminoethyl
Particle size: 45-165 µm
BSA adsorption capacity:>80 mg/mL
Ion-exchange capacity: 0.12-0.17 mmol/mL
Maximum flow rate: 750 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-30°C
Long-term pH stability: 2-12
Short-term CIP pH stability: 1-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min

Weak DEAE selectivity
>80 mg/mL BSA capacity
Up to 750 cm/h flow
Low operating backpressure
Broad CIP pH tolerance
Steam-resistant matrix
Low nonspecific adsorption
Scalable biomolecule purification

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