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Conventional PCR (CPCR) (CAT#: STEM-MB-0184-WXH)
Introduction
The polymerase chain reaction (PCR) is a test tube system for DNA replication which allows a “target” DNA sequence to be selectively amplified several million folds in just a few hours. The PCR achieves amplification of a predetermined fragment of DNA, (the target; which can, e.g., be from 100 to 1000 bp long)
Principle
The PCR technique is based on the enzymatic replication of DNA. In PCR, a short segment of DNA is amplified using primer mediated enzymes. DNA Polymerase synthesises new strands of DNA complementary to the template DNA. The DNA polymerase can add a nucleotide to the pre-existing 3'-OH group only.
Applications
• DNA sequencing and gene reproduction & cloning.
• Cellular cloning.
• Identification of genetic fingerprints.
• Discovering the possibility of transmitting genetic diseases between potential parents and determining whether the diseases will or will not be passed to their next generation of children.
• DNA-based phylogeny, or functional analysis of genes.
• The diagnosis of hereditary diseases.
• The detection and diagnosis of infectious diseases.
• Cellular cloning.
• Identification of genetic fingerprints.
• Discovering the possibility of transmitting genetic diseases between potential parents and determining whether the diseases will or will not be passed to their next generation of children.
• DNA-based phylogeny, or functional analysis of genes.
• The diagnosis of hereditary diseases.
• The detection and diagnosis of infectious diseases.
Procedure
1.Initialization
2.Denaturation
3.Annealing
4.Extension/elongation
2.Denaturation
3.Annealing
4.Extension/elongation
Materials
What are the 5 key basic reagents used in PCR?
Generally, a complete polymerase chain reaction (PCR) requires five basic reagents, including DNA template, forward and reverse primers, DNA polymerase, deoxynucleotide triphosphates (dNTPs) and reaction buffer.
Generally, a complete polymerase chain reaction (PCR) requires five basic reagents, including DNA template, forward and reverse primers, DNA polymerase, deoxynucleotide triphosphates (dNTPs) and reaction buffer.
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Wide range of applications
For various laboratory needs
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