Collision Cross Section Conformational Analyses of Bile Acids by Ion Mobility–Mass Spectrometry (CAT#: STEM-ST-0179-LJX)

Introduction

Bile acids serve as one of the most important classes of biological molecules in the gastrointestinal system. Due to their structural similarity, bile acids have historically been difficult to accurately annotate in complex biological matrices using mass spectrometry. They often have identical or nominally similar mass-to-charge ratios and similar fragmentation patterns that make identification by mass spectrometry arduous, normally involving chemical derivatization and separation via liquid chromatography. Here, we demonstrate the use of drift tube ion mobility (DTIM) to derive collision cross section (CCS) values in nitrogen drift gas (DTCCSN2) for use as an additional descriptor to facilitate expedited bile acid identification.