Co-NTA Resin, 100ml Resin, 786-933, G-BIOSCIENCES (CAT#: STEM-C-3152-LGZ)

Immobilized metal ion affinity chromatography (IMAC) was developed by Porath (1975) and is based on the association of certain protein residues (histidine, cysteine and to some extent tryptophan) with transition metals cation interactions.

Co-NTA resins are specifically designed for the purification of recombinant proteins fused to 6 x histidine (6XHis) tags expressed in bacterial, insect and mammalian cells. The resin has high affinity and selectivity for recombinant fusion proteins tagged with 6 tandem histidine residues. Although the binding efficiency of 6X His-tagged proteins is lower compared to nickel-chelating resins, non-specific binding is significantly reduced.

This Co-NTA resin can be used to purify 6X His-tagged proteins under native and denaturing conditions. Resin-bound proteins can be competitively eluted with low pH buffers or with imidazole or histidine.

Co-NTA resin uses nitric acid triacetic acid (NTA), a tetra-acid chelating ligand, in a highly cross-linked 6% agarose matrix. NTA binds Co2+ ions through four coordinations.

The resin has a capacity of 20-40 μmol Co2+/ml resin. The protein binding capacity is 50 mg protein per ml resin. We have demonstrated the binding of 100mg of 50kDa 6XHis-tagged protein to one milliliter of resin.

The resin bed volumes of the spin columns were 0.2, 1 and 3 ml, respectively, and the total column volumes were 1, 8 and 22 ml, respectively. The column is available as a spin format for gravity flow columns.

Can also process up to 96 samples in a 96-well spin plate format.

Immobilized IDA nickel, copper, cobalt, zinc and nickel nta chelating resins are also available. Cobalt has the highest selectivity, followed by zinc, nickel, and copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by nickel and then zinc.

Specific binding/wash and elution buffers are available.

Our new HOOK™ 6X His Protein Purification Kit is now available and includes everything needed to purify 6X His-tagged proteins from yeast or bacteria, including lysis buffer, lysing enzyme, resin, column and binding, wash and elution buffers . Each kit is available with nickel or cobalt chelate resins. Available kits are:

HOOK™ 6X His Protein Purification (Bacteria): Isolated from bacteria; optimized to produce up to 10mg/250ml of soluble 6X His-tagged protein.
HOOK™ 6X His Protein Spin Purification (Bacteria): Isolation from bacteria; optimized to produce ~1mg/50ml of soluble 6X His-tagged protein culture
HOOK™ 6X His Protein Purification (Yeast): Isolated from yeast; optimized to yield up to 10 mg of soluble 6X His-tagged protein.
HOOK™ 6X His Protein Spin Purification (Yeast): Isolated from yeast; optimized to yield up to 1 mg of soluble 6X His-tagged protein.

Size: 100ml Resin

Uses nitrilotriacetic acid (NTA), a tetradenate chelating ligand
For the purification of 6x His proteins
High capacity: >50mg/ml
Ligand density: 20-40μmoles Co2+ /ml resin
Bead Structure: 6% cross-linked agarose

Carbohydrate Coupling Resin, 10ml Resin, 786-808, G-BIOSCIENCES (CAT#-STEM-C-3112-LGZ)
HOOK™ Activated Agarose (Amine Reactive), 10ml Resin, 786-066, G-BIOSCIENCES (CAT#-STEM-C-3113-LGZ)
Immobilized O-Phosphorylethanolamine, 5 ml, 6% Cross-Linked Agarose, 786-1310, G-BIOSCIENCES (CAT#-STEM-C-3114-LGZ)
Sulfhydryl Coupling Resin, 10ml Resin, 786-794, G-BIOSCIENCES (CAT#-STEM-C-3115-LGZ)
Sulfhydryl Coupling Resin, 50ml Resin, 786-795, G-BIOSCIENCES (CAT#-STEM-C-3116-LGZ)
Sulfhydryl Coupling Resin, 250ml Resin, 786-796, G-BIOSCIENCES (CAT#-STEM-C-3117-LGZ)