Characterization of nanomaterials by Raman Spectroscopy (CAT#: STEM-ST-0025-WXH)

Introduction

Raman spectroscopy undoubtedly emerged as a relevant tool for probing and characterization of nanomaterials like nanosensor, nanotube and nanowire, as these are intensively studied in various areas of science due to their unique mechanical, electrical and chemical properties. For better understanding of nanomaterials like nanoceramic, nanocomposite, glassy material and semiconductor, techniques like micro Raman spectroscopy have been successfully applied for their better characterization.

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Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope
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