Characterization of ion channels in endolysosomes by Patch clamp (CAT#: STEM-PET-0029-WXH)

Introduction

Endolysosomes are involved in a wide range of cellular processes including membrane trafficking, catabolism of extracellular and intracellular components, immune responses and antigen presentation, cell secretions, and cell life and death <br />The endolysosomal ion channels regulate the function of the endolysosomal system through four basic mechanisms: calcium release, control of membrane potential, pH change, and osmolarity regulation.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

The patch-clamp technique involves a glass micropipette forming a tight gigaohm seal with the cell membrane. The micropipette contains a wire bathed in an electrolytic solution to conduct ions. To measure single ion channels, a “patch” of membrane is pulled away from the cell after forming a gigaohm seal.

Applications

• Study of ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane.
• Study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells.
• Study of ion channels.

Procedure

1. Fabrication of glass electrodes
2. Measuring glass electrode resistance and compensating offset potential
3. Glass electrode contact to cell membrane and obtain a GΩ seal
4. Acquire and analyse recordings using the appropriate software.

Materials

Patch clamp system

Other recommended products

Study of the odorant receptor MOR23 by Patch clamp (CAT#: STEM-PET-0058-WXH)
Study of substantia gelatinosa by Patch clamp (CAT#: STEM-PET-0046-WXH)
In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem by Patch clamp (CAT#: STEM-PET-0072-WXH)
Analysis of dopaminergic antinociceptive actions on substantia gelatinosa neurons in the spinal cord by Patch clamp (CAT#: STEM-PET-0066-WXH)
Analysis of granule cells of rat hippocampus by Patch clamp (CAT#: STEM-PET-0073-WXH)
Characterization of Anion Channels by Patch clamp (CAT#: STEM-PET-0051-WXH)
Microcircuit analysis of individual human brains by Patch clamp (CAT#: STEM-PET-0033-WXH)
Study of Ion channels in the thylakoid membrane by Patch clamp (CAT#: STEM-PET-0056-WXH)