Characterization of Acinetobacter baumannii ZnuA by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0829-WXH)

Introduction

Acinetobacter baumannii is a Gram-negative nosocomial pathogen associated with significant disease. Crucial to the survival and pathogenesis of A. baumannii is the ability to acquire essential micronutrients such as Zn(II). Recruitment of Zn(II) by A. baumannii is mediated, at least in part, by the periplasmic solute-binding protein ZnuA and the ATP-binding cassette transporter ZnuBC.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Differential Scanning Fluorimetry measures protein thermal unfolding by monitoring changes in fluorescence emission of a sample upon heating. This allows the determination of protein thermostability and complex formation even with weakly binding ligands by thermal shift assay. Differential Scanning Fluorimetry is therefore ideally suited for screening of optimum buffer conditions like pH, buffer composition and ionic strength. The technique is applicable to any biological sample, from soluble proteins to integral membrane proteins.

Applications

To identify low-molecular-weight ligands that bind and stabilize purified proteins.
To measure the denaturation and unfolding of proteins.

Procedure

1. Preparation of compound solutions
2. Preparation of buffer/additive screen plates
3. Preparation of compound storage plates
4. Equipment preparation
5. Sample preparation
83. Performing the scan

Materials

Real-time PCR instrument

Other recommended products

Analysis of Staphylococcus aureus FhuD2 by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0830-WXH)
Analysis of Disease-Related Misfolded Proteins by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0790-WXH)
Identification of inhibitors of protein-protein interactions by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0779-WXH)
Identification of nuclear receptor ligands by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0768-WXH)
Analysis of lac operon by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0812-WXH)
Analysis of Glucose-binding proteins (GBPs) by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0828-WXH)
Characterization of aminoacyl-tRNA (AARS) synthetase stability by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0769-WXH)
Analysis of carbonic anhydrase by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0758-WXH)