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Characteristics of porphyrin-DNA complexes by Linear dichroism (LD) (CAT#: STEM-ST-0125-WXH)

Introduction

Porphyrins are a group of heterocyclic macrocycle organic compounds, composed of four modified pyrrole subunits interconnected at their α carbon atoms via methine bridges (=CH-). In vertebrates, an essential member of the porphyrin group is heme, which is a component of hemoproteins, whose functions include carrying oxygen in the bloodstream. In plants, an essential porphyrin derivative is chlorophyll, which is involved in light-harvesting and electron transfer in photosynthesis.




Principle

Linear dichroism (LD) or diattenuation is the difference between absorption of light polarized parallel and polarized perpendicular to an orientation axis. It is the property of a material whose transmittance depends on the orientation of linearly polarized light incident upon it. LD measurements are based on the interaction between matter and light and thus are a form of electromagnetic spectroscopy.

Applications

Study the functionality and structure of molecules.

Materials

• Polarizer
• Absorbance spectrometer