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CellTiter-Glo (CTG) for cell viability assays (CAT#: STEM-CBT-0004-WXH)

Introduction

CellTiter-Glo (CTG) is a rapid cell viability assay based on ATP detection. It is a recognized high-sensitivity luminescent assay and the gold standard for cell viability assays. ATP adenosine triphosphate participates in various enzymatic reactions in organisms and is an indicator of the metabolism of living cells. Its content directly reflects the number and state of cells. During the experiment, an equal volume of CTG reagent was added to the cell culture medium to measure the luminescence value. In the light signal and system, the luminescence value is proportional to the amount of ATP, and ATP is positively correlated with the number of living cells. Therefore, the cell can be obtained by detecting the ATP content. vitality.




Applications

This system has been widely used in the field of life science research, such as the activity detection of some biologically active factors, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity determination, etc.

Procedure

1. Cell Treatment
2. CellTiter-Glo detection solution incubation
3. Add the reaction solution
4. OD value determination

Notes

Customer provided:
1. Cell type and name
2. Detection time node

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