Surface Plasmon Resonance (SPR) Technology

Surface plasmon resonance (SPR) spectroscopy is a powerful, label-free technique to monitor noncovalent molecular interactions in real time and in a noninvasive fashion. As a label-free assay, SPR does not require tags, dyes, or specialized reagents (e.g., enzymes–substrate complexes) to elicit a visible or a fluorescence signal. SPR biosensing appears to be one of the most powerful approach for monitoring of affinity binding of biomolecules, and primary screening of druggable molecules. SPR-type sensors are increasingly used to study a variety of biological entities, such as DNAs, RNAs, proteins, carbohydrates, lipids, and cells in the field of biomedical research. SPR has been used as a powerful tool to study interactions between biomolecules, including antibody-antigen, ligand-receptor kinetics, and enzyme-substrate reaction.