Carboxyl Magnetic Beads, 5ml Resin, 786-909, G-BIOSCIENCES (CAT#: STEM-C-3094-LGZ)

G-Biosciences' carboxyl magnetic beads are 1 micron homogeneous magnetic beads with surface functional group -COOH. The magnetic beads consist of a single crystal Fe3O4 core and a dextran coating. By chemically modifying dextran, the carboxyl group (-COOH) is attached to the magnetic beads through a short hydrophilic linker. The hydrophilic surface ensures the excellent dispersibility and easy handling of the magnetic beads in various buffers.

Molecules can be coupled to free amines via a variety of amine reaction methods; however, the use of carbodiimide EDCs allows coupling of free carboxyl groups. The resulting amide bond is highly stable, greatly reducing the chance of leaching of the affinity tag.

Carbodiimide activation of the carboxyl group produces a very unstable intermediate that undergoes rapid hydrolysis, implying that the ligand needs to be added rapidly. Alternatively, a two-step protocol using n-hydroxysuccinimide (NHS) can be used to produce unstable intermediates with longer reaction times.

Size: 5ml resin

1µm beads
~0.2mM ligand density
Binds rapidly at neutral to high pH
Compatible with a variety of carboxyl-reactive crosslinking reagents
No need for centrifugation
Dextran coating for low non-specific binding

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