Canine Influenza Virus H3N2 Subtype Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1028-ZJF)

This assay kit applies SYBR Green dye-based real-time PCR to amplify and detect Canine Influenza Virus H3N2 Subtype nucleic acids. Specific primers are designed to target conserved regions of the target gene. Fluorescence signals emitted during amplification are recorded in real time, supporting accurate identification and measurement of Canine Influenza Virus H3N2 Subtype in experimental and routine laboratory settings.

Kit Components:
Canine Influenza Virus H3N2 Subtype Amplification Mix: 50 µL × 1 tube
Canine Influenza Virus H3N2 Subtype Reaction Mix: 950 µL × 1 tube
Canine Influenza Virus H3N2 Subtype Positive Control: 50 µL × 1 tube
Canine Influenza Virus H3N2 Subtype Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Convenient direct-use format with only template DNA input needed.
Primer sequences selected for unmatched specificity to the analyte.
Improved detection limit due to optimized assay chemistry.
Internal positive control enables result interpretation.
Enclosed qPCR protocol limits exposure and contamination.
Not intended for diagnostic or therapeutic use.

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