This butyl hydrophobic interaction medium is designed for purification of hepatitis B vaccine products. It uses a four-percent agarose matrix functionalized with butyl groups. Hydrophobic interactions enable selective binding of target biomolecules under elevated salt conditions. Lowering the salt concentration releases bound components according to hydrophobic strength. The spherical particles support consistent column packing and reproducible operation. Protein adsorption above 26 mg HSA/mL provides useful preparative capacity. The medium operates at 150-250 cm/h under the stated pressure condition. Broad pH and chemical stability support repeated cleaning and regeneration. It can tolerate steam treatment under the specified saline conditions.
Specification
Model: Butyl Seplife 4FF Appearance: White spherical gel Matrix: Seplife 4FF Ligand: Butyl Shape: Spherical Particle size: 45-165 µm Pressure-flow range: 150-250 cm/h at 0.15 MPa Protein adsorption capacity:>26 mg HSA/mL Operating temperature: 4-40°C Long-term pH stability: 3-13 Short-term CIP pH stability: 2-14 Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 70% ethanol, 50% ethylene glycol, 8 M urea, 6 mol/L guanidine hydrochloride, and 1 mM hydrochloric acid Heat resistance: 121°C in 0.1 M sodium chloride for 30 min
Features
Designed for hepatitis B vaccine purification Butyl hydrophobic ligand >26 mg/mL protein capacity Steam-resistant agarose matrix Broad chemical compatibility Wide CIP pH tolerance Reproducible spherical particles Moderate process flow