Butyl Seplife 4FF Hydrophobic Interaction Medium,>26 mg HSA/mL (CAT#: STEM-BC-3136-ZJF)

Highlights

Designed for hepatitis B vaccine purification
Butyl hydrophobic ligand
>26 mg/mL protein capacity

Cat Number: STEM-BC-3136-ZJF

Application: for hepatitis B vaccine hydrophobic purification

Model: Butyl Seplife 4FF




Description

This butyl hydrophobic interaction medium is designed for purification of hepatitis B vaccine products. It uses a four-percent agarose matrix functionalized with butyl groups. Hydrophobic interactions enable selective binding of target biomolecules under elevated salt conditions. Lowering the salt concentration releases bound components according to hydrophobic strength. The spherical particles support consistent column packing and reproducible operation. Protein adsorption above 26 mg HSA/mL provides useful preparative capacity. The medium operates at 150-250 cm/h under the stated pressure condition. Broad pH and chemical stability support repeated cleaning and regeneration. It can tolerate steam treatment under the specified saline conditions.

Specification

Model: Butyl Seplife 4FF
Appearance: White spherical gel
Matrix: Seplife 4FF
Ligand: Butyl
Shape: Spherical
Particle size: 45-165 µm
Pressure-flow range: 150-250 cm/h at 0.15 MPa
Protein adsorption capacity:>26 mg HSA/mL
Operating temperature: 4-40°C
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 70% ethanol, 50% ethylene glycol, 8 M urea, 6 mol/L guanidine hydrochloride, and 1 mM hydrochloric acid
Heat resistance: 121°C in 0.1 M sodium chloride for 30 min

Features

Designed for hepatitis B vaccine purification
Butyl hydrophobic ligand
>26 mg/mL protein capacity
Steam-resistant agarose matrix
Broad chemical compatibility
Wide CIP pH tolerance
Reproducible spherical particles
Moderate process flow

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