Bordetella Pertussis Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1090-ZJF)

Utilizing SYBR Green real-time PCR technology, this detection kit enables the amplification and quantification of Bordetella Pertussis with high efficiency. Specific primers are designed to bind to conserved gene regions of the target. As PCR progresses, fluorescence is generated and captured by the system in real time, providing clear and reliable interpretation of the presence and quantity of Bordetella Pertussis in test samples.

Kit Components:
Bordetella Pertussis Amplification Mix: 50 µL × 1 tube
Bordetella Pertussis Reaction Mix: 950 µL × 1 tube
Bordetella Pertussis Positive Control: 50 µL × 1 tube
Bordetella Pertussis Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Easy-start kit: DNA template is the only requirement.
Conserved-region targeting ensures reliable specificity.
Optimized formulation supports high-sensitivity detection.
Positive sample control included to validate results.
Self-contained PCR setup prevents external contamination.
Restricted to experimental research usage.

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