Blue Seplife FF Dye-Affinity Medium,>18 mg BSA/mL (CAT#: STEM-BC-3143-ZJF)

This dye-affinity chromatography medium is designed for purification of albumin and selected proteins. It uses a high-flow agarose matrix coupled with Cibacron Blue 3G-A ligand. Protein binding results from combined electronic and hydrophobic interactions with the dye ligand. The medium can capture albumin, interferons, coagulation factors, and enzymes requiring NAD+ or NADP+. Spherical particles support uniform bed formation and reproducible flow. A binding capacity above 18 mg BSA/mL supports preparative purification. The medium tolerates selected denaturants and ethanol. It can also withstand the stated heat-treatment conditions in water.

Model: Blue Seplife FF
Appearance: White spherical particles, odorless and tasteless
Matrix: Seplife 6FF
Ligand: Cibacron Blue 3G-A
Particle size: 45-165 µm
Maximum flow rate: 750 cm/h
Ligand density: 7 µmol/mL
Binding capacity:>18 mg BSA/mL
Long-term pH stability: 3-13
Short-term CIP pH stability: 4-12
Chemical compatibility: 6 M guanidine hydrochloride, 8 M urea, and 70% ethanol
Heat resistance: 121°C in water for 20 min
Applications: Albumin, interferons, coagulation factors, and NAD+/NADP+-dependent enzymes

Cibacron Blue affinity ligand
>18 mg/mL BSA capacity
Up to 750 cm/h flow
Suitable for albumin purification
Captures nucleotide-dependent enzymes
Denaturant-compatible matrix
Heat-resistant medium
Low ligand loss

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