Blastocystis Giardia Flagellate Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2097-ZJF)

This real-time PCR kit is designed for the sensitive and specific detection of Blastocystis Giardia Flagellate using probe-based technology. Primers targeting conserved gene regions of Blastocystis Giardia Flagellate ensure precise amplification. The generated fluorescence is continuously tracked throughout the reaction, enabling reliable qualitative and quantitative diagnostics.

Kit Components:
Blastocystis Giardia Flagellate Amplification Mix: 50 µL × 1 tube
Blastocystis Giardia Flagellate Reaction Mix: 950 µL × 1 tube
Blastocystis Giardia Flagellate Positive Control: 50 µL × 1 tube
Blastocystis Giardia Flagellate Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

User-friendly design with ready-to-go reagent mix.
Conserved-region primer/probe sets ensure accurate targeting.
Sensitivity enhanced through meticulous component tuning.
Supplied with a control sample to confirm test results.
Fully enclosed PCR system lowers contamination probability.
Rapid amplification process shortens test duration.
Suitable only for non-clinical laboratory use.

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