Bear-Derived Component Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0040-ZJF)

This real-time PCR kit incorporates SYBR Green dye chemistry for the detection ofBear-Derived Component. With primers tailored to conserved gene regions, the reaction generates fluorescence during amplification. The system monitors this signal continuously, allowing users to obtain precise qualitative and quantitative data from various sample types.

Kit Components:
Bear-Derived Component Amplification Mix: 50 µL × 1 tube
Bear-Derived Component Reaction Mix: 950 µL × 1 tube
Bear-Derived Component Positive Control: 50 µL × 1 tube
Bear-Derived Component Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use kit requiring only user-supplied DNA template.
High specificity: Primers are designed based on highly conserved regions of bear-derived DNA and do not amplify non-bear components.
Optimized primers and amplification system provide sensitivity 100 times greater than conventional PCR.
Includes non-infectious PCR positive control to distinguish false-negative results.
Closed one-tube fluorescence PCR operation reduces the risk of environmental contamination.
For research use only; kit contains sufficient reagents for 50 PCR reactions using a 2 μL system.

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