Axon Axoporator 800A Electroporator, Molecular Devices, LLC (CAT#: STEM-LE-0522-LC)

The Axoporator 800A Electroporator is a molecular delivery system based on a micropipette. It uses voltage pulses to cause transient formation of membrane pores through which impermeable molecules and ions that penetrate the membrane can enter cells. The mechanism is the same as that used for the overall electroporation of suspended cells-the dielectric breakdown of the membrane by voltage pulses. However, single-cell electroporation has obvious advantages over bulk electroporation: individual cells can target specific modifications. Whether you are using isolated cells, tissue sections, or complete preparations, this technology allows you to focus on individual cells.

Voltage Range:100 to 264 V (AC), 12 to 14 V (DC)

No computer is required. Microscope, micromanipulator and micropipette are all you need to complete the system.
Small footprint, easy to place near the microscope
Three different pulse types that can be configured at any polarity to ensure the best waveform for your application
Pulse sequence maximizes the flexibility of the voltage waveform
Automatic and manual counters simplify tracking of the number of electroporation commands.
The voltage range of up to 100 V provides the best voltage for single-cell electroporation, which can be as high as tens of volts in some preparations.
Available AP-1A-0.1MU probe with current passing range up to ±100 µA
The built-in audio monitor with headphone jack changes the tone when you are near the battery and touching it.
The combination of a resistance display and an audio monitor provides a useful tool to search for cells in tissue sections without visual control.
Independent or computer-controlled pulse activation provides maximum flexibility and synchronization with electrophysiology experiments. For more details, please see the "Related Products" section
When the foot is busy, the foot switch can trigger the pulse to start
Synchronous output BNC to synchronize third-party recording system
The user guide written by the scientific consultant, with the assistance of Axon Instruments employees, can be directly launched and used as an in-depth reference.