Avian Enteropathogenic E. coli O1 Serotype Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2482-ZJF)

This assay kit uses probe-based real-time PCR to amplify and detect Avian Enteropathogenic E. coli O1 Serotype. The workflow includes template amplification, signal generation, and real-time fluorescence tracking, delivering robust qualitative and quantitative data with high specificity and minimal background interference.

Kit Components:
Avian Enteropathogenic E. coli O1 Serotype Amplification Mix: 50 µL × 1 tube
Avian Enteropathogenic E. coli O1 Serotype Reaction Mix: 950 µL × 1 tube
Avian Enteropathogenic E. coli O1 Serotype Positive Control: 50 µL × 1 tube
Avian Enteropathogenic E. coli O1 Serotype Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Ready-to-use format; users only need to provide sample DNA.
Primers and probes are designed from conserved regions for high specificity.
Optimized oligo sequences deliver excellent sensitivity.
Positive control is included to help identify false-negative results.
Closed-tube, single-step workflow minimizes contamination risk.
Fast detection with minimal processing time.
For research use only.

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