Asymmetric PCR (CAT#: STEM-MB-0192-WXH)

Introduction

Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. The technique has applications in some types of sequencing and hybridization probing where having only one of the two complementary strands is required. Asymmetric PCR differs from regular PCR by the excessive amount of primers for a chosen strand. Due to the slow (arithmetic) amplification later in the reaction (after the limiting primer has been used up) extra cycles of PCR are required.<br />Asymmetric PCR is mainly used to prepare ssDNA for sequencing, which has the advantage that the remaining primers do not have to be removed before sequencing because the small amount of restriction primers has been exhausted. Most scholars believe that DNA sequence analysis using cDNA by asymmetric PCR is a good method for studying eukaryotic DNA exons.<br />Linear-after-the-exponential (LATE)-PCR describes a novel approach to asymmetric PCR which uses adjusted melting temperatures of the limited primer to increase PCR efficiency.