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Assessment of Pearl Quality by UV-Vis Spectroscopy (CAT#: STEM-MB-0900-WXH)

Introduction

Pearl quality is determined by five primary factors: lustre, color, shape, surface contour and size. Many of these characteristics are subjective and may depend on individual perceptions. Pearl grading is also labor intensive and requires skill and experience. A pearl grader has to be able to quantify and collate all visual observations and allocate each pearl to a defined grading level. However, techniques are available that may assist in reducing subjectivity in assessing some aspects of pearl quality. As UV-Vislight passes through, or is reflected by, a material, specific groups of wavelengths are absorbed and the remaining light is reflected and interpreted by the eye as color. Based on the wavelengths detected within the ultraviolet and eye-visible regions, UV-Vis spectroscopy can rapidly identify chemical properties and color pigments in a non-destructive way. Because pearl color and overtone is determined by the way in which light is reflected through the various outer layers of nacre forming the pearl, this technique has obvious potential application in quantification of pearl color.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer